HBB Mouse Monoclonal Antibody [Clone ID: PLA114]

CAT#: TA363877

Hemoglobin beta (Beta-globin), mouse anti porcine, clone PLA114

Product Images

TA363877, Clone PLA-114, swine ileum, paraffin section

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  TA363877, Clone PLA-114, swine ileum, paraffin section

Specifications

Product Data

• Clone Name: PLA114
• Applications: ELISA, IHC
• Recommended Dilution: Tested for immunohistochemistry (IHC) and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.2µg/ml (1:2000) IHC, paraffin sections: 10µg/ml (1:40) Western Blot: 0.25µg/ml (1:2000) Optimal dilutions should be determined by the end user.
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Porcine liver extract.
• Specificity: Pig: Erythrocytes, plasma Other species: not tested
  
  Epitope: Immunoprecipitation and subsequent identification by nanoLC- ESI-MS/MS identified the antigen as hemoglobin beta chain. The epitope has not been further characterized.
  
  Distribution: Tissue sections: In paraffin sections the antibody stains all erythrocytes whereas in frozen sections the antibody yields a picture typical of a molecule leaking out of blood vessels and diffusing into surrounding tissue.
• Formulation: Affinity purified from cell culture supernatant, lyophilized. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: 1 year at 4° - 8°C. Avoid repeated thawing and freezing of the reconstituted antibody.
• Database Link:
  UniProt ID P02067
• Background: Monoclonal antibody PLA114 is specifically directed against the porcine hemoglobin beta subunit. Hemoglobin contains four subunits, of which two are identical. They form the α2β2 scaffold that holds the iron-containing heme group which is responsible for oxygen transport by red blood cells. Each subunit has a molecular weight of about 16kDa. In humans, the beta-globin gene is activated around the time of birth, replacing embryonic and fetal globin genes. In humans, mutations in the adult β-globin gene cause β-thalassemia and sickle cell disease (SCD). β-Thalassemia results from a reduced production of β-globin while SCD is caused by a mutation that results in an amino acid substitution of adult β-globin. Hemoglobin tetramers bearing this mutation polymerize inside red blood cells and distort them into a characteristic crescent-shaped appearance. These rigid sickle cells are more prone to lysis and tend to occlude blood flow in the microvasculature, causing severe physiological problems. This antibody was produced serum-free, without fetal calf serum.
• Synonyms: beta-globin; CD113t-C; HBD