ATP4A Monoclonal Antibody [Clone ID: PPC19]

CAT#: TA363882

H,K-ATPase alpha, (ATP4A), mouse anti porcine, clone PPC19

Product Images

TA363882, Clone PPC19, swine stomach, paraffin section

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  TA363882, Clone PPC19, swine stomach, paraffin section

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  TA363882, Clone PPC19, swine stomach, paraffin section

Specifications

Product Data

• Clone Name: PPC19
• Applications: ELISA, IHC, WB
• Recommended Dilution: Each lot of this antibody has been tested and validated for immunohistochemistry on formalin-fixed paraffin sections (IHC-p). Approximate working dilution for IHC: Paraffin sections: 4-8µg/ml (1:50-1:100); microwave pretreatment for antigen retrieval is recommended.
• Reactivities: Pig
• Clonality: Monoclonal
• Immunogen: Porcine stomach extract.
• Specificity: Pig: parietal (oxyntic) cells Other: not tested
  
  Epitope: The antibody reacts with formaldehyde-treated porcine gastric H,K-ATPase. The epitope has not been further characterized.
  
  Distribution: Tissue sections: porcine gastric parietal (oxyntic) cells of the fundic gland region
• Formulation: Affinity purified from cell culture supernatant, lyophilized, packaged under nitrogen. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 6mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: 1 year at 4° - 8°C. Minimize repeated thawing and freezing of the stock solution.
• Database Link:
  Entrez Gene 397552 Pig
  UniProt ID P19156
• Background: Monoclonal antibody PPC19 recognizes the porcine gastric H,K-ATPase. This enzyme catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane and is thus responsible for acid production in the stomach. The functional proton pump is actually a heterodimer consisting of a 114kDa alpha subunit with catalytic activity, and a 33kDa stabilizing beta subunit. Of the various cell types that form the gastric mucosa, only parietal (oxyntic) cells of the fundic gland region possess this enzyme. Of pathological significance, their dysfunction induced by H. pylori infection leads to atrophic gastritis. This antibody was produced in vitro in bioreactors.
• Synonyms: ATP6A