calc1 Rabbit Polyclonal Antibody

CAT#: TA363892

Calcitonin (salmon I); diluted antiserum

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against Calcitonin. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide H-Cys-Ser-Asn-Leu-Ser-Thr-Cys-Val-Leu-Gly-Lys- Leu-Ser-Gln-Glu-Leu-His-Lys-Leu-Gln-Thr-Tyr-Pro-Arg-Thr-Asn-Thr-Gly- Ser-Gly-Thr-Pro-NH2, (Disulfide bond) coupled to carrier protein.
• Formulation: This polyclonal antibody is supplied as a lyophilized powder. The powder should be rehydrated with 50ml of RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4° - 8°C. This should ensure antibody stability for approximately one month.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Database Link:
  UniProt ID P01263
• Background: Calcitonin is a 32 amino acid peptide hormone that defines the calcitonin-like protein family. It is formed by the proteolytic cleavage of a larger prepropeptide, which is the product of the CALC1 gene and secreted by parafollicular cells of the thyroid gland in humans, and in the ultimopharyngeal body in animals. The hormone participates in calcium and phosphorus metabolism by reducing blood calcium, and counteracts the effects of parathyroid hormone and Vitamin D3. This antibody was generated by immunization of rabbits with Calcitonin to a carrier protein.