Bombesin Rabbit Polyclonal Antibody

CAT#: TA364013

Rabbit polyclonal anti Bombesin; diluted antiserum

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against Bombesin. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Reactivities: Human, Mammalian
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide Pyr-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His- Leu-Met-NH2 coupled to carrier protein.
• Formulation: Each vial contains enough antiserum for 500 RIA tubes. The powder should be rehydrated with 50ml RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4°C. This should ensure antibody stability for approximately one month.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Background: Bombesin is a 14-amino acid peptide originally isolated from the skin of the European fire- bellied toad (Bombina bombina). It has two known homologs in mammals called neuromedin B and gastrin-releasing peptide. Bombesin stimulates gastrin release from G cells and activates three different G-protein-coupled receptors known as BBR1, -2, and -3. It also activates these receptors in the brain. Together with cholecystokinin, Bombesin is the second major source of negative feedback signals that stop eating behaviour. Also, it is known to be a tumor marker for small cell carcinoma of lung, gastric cancer, pancreatic cancer, and neuroblastoma. This antibody was generated by immunization of rabbits with Bombesin coupled to a carrier protein.