Guca2a Rabbit Polyclonal Antibody

CAT#: TA364245

Rabbit polyclonal anti Guanylin (rt); purified rabbit IgG

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against Guanylin. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Formulation: Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Gene Name: guanylate cyclase activator 2A
• Database Link:
  Entrez Gene 25656 Rat
  UniProt ID P28902
• Background: Guanylin is a 15 amino acid polypeptide that is secreted by goblet cells in the colon. It acts as an agonist of the guanylyl cyclase receptor GC-C and regulates electrolyte and water transport in intestinal and renal epithelia. Upon receptor binding, guanylin increases the intracellular concentration of cGMP, induces chloride secretion and decreases intestinal fluid absorption, ultimately causing diarrhoea. The peptide stimulates the enzyme through the same receptor binding region as the heat-stable enterotoxins. This antibody was generated by immunization of rabbits with Guanylin coupled to a carrier protein.
• Synonyms: Gap-I; GUANYLIN; GUCA2; STARA