Parathyroid Hormone (PTH) Rabbit Polyclonal Antibody

CAT#: TA364251

Rabbit polyclonal anti (Asp76)-pTH (39-84) (hu); diluted antiserum

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against (Asp76)- Parathyroid Hormone (pTH) (39-84). Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide H-Ala-Pro-Leu-Ala-Pro-Arg-Asp-Ala-Gly-Ser-Gln-Arg- Pro-Arg-Lys-Lys-Glu-Asp-Asn-Val-Leu-Val-Glu-Ser-His-Glu-Lys-Ser- Leu-Gly-Glu-Ala-Asp-Lys-Ala-Asp-Val-Asp-Val-Leu-Thr-Lys-Ala-Lys-Ser- Gln-OH coupled to carrier protein.
• Formulation: Each vial contains enough antiserum for 500 RIA tubes. The powder should be rehydrated with 50ml RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4°C. This should ensure antibody stability for approximately one month.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Gene Name: parathyroid hormone
• Database Link:
  Entrez Gene 5741 Human
  UniProt ID P01270
• Background: Parathyroid hormone (PTH) is a single polypeptide of 84 amino acids. It is a critical hormone in the regulation of Ca2+ homeostasis and secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium, and promote renal tubular reabsorption of calcium and increased renal excretion of phosphates. It is a major regulator of bone metabolism. Secretion of parathyroid hormone increases when the level of calcium in the extracellular fluid is low. [Asp76] hPTH-(39-84) inhibited the response to intact PTH to a lesser extent, whereas [Asp76] hPTH-(53-84) had no antagonistic activity. This antibody was generated by immunization of rabbits with (Asp76)-Parathyroid Hormone (pTH) (39-84) coupled to a carrier protein.
• Synonyms: parathormone; Parathyrin; PTH1