Parathyroid Hormone (PTH) Rabbit Polyclonal Antibody

CAT#: TA364262

Rabbit polyclonal anti pTH (7-84) (hu); purified rabbit IgG

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against pTH (7- 84). Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide H-Leu-Met-His-Asn-Leu-Gly-Lys-His-Leu-Asn-Ser- Met-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-is-Asn-Phe- Val-Ala-Leu-Gly-Ala-Pro-Leu-Ala-Pro-Arg-Asp-Ala-Glu-Ser-Gln-Arg-Pro- Arg-Lys-Lys-Glu-Asp-Asn-Val-Leu-Val-Glu-Ser-His-Glu-Lys-Ser-Leu- Gly-Glu-Ala-Asp-Lys-Ala-Asp-Val-Asn-Val-Leu-Thr-Lys-Ala-Lys-Ser-Gln- OH coupled to a carrier protein.
• Formulation: Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Gene Name: parathyroid hormone
• Database Link:
  Entrez Gene 5741 Human
  UniProt ID P01270
• Background: Human Parathyroid Hormone (PTH) is a hormone expressed and secreted as prepro-PTH by chief cells of the parathyroid glands when extracellular calcium levels are low. PTH is coexpressed with furin, the enzyme believed to be responsible for processing pro-PTH to PTH. PTH is secreted as a C-terminal fragment (7-84) when extracellular calcium levels are high, offsetting the effects of pTH. It acts as an antagonist for PTH1R, as well as a possible carboxy-terminus interacting pTH receptor. PTH and truncated pTH fragments play a major role in bone metabolism and calcium homeostasis, as well as renal excretion. This antibody was generated by immunization of rabbits with pTH (7-84) coupled to a carrier protein.
• Synonyms: parathormone; Parathyrin; PTH1