EDN1 Rabbit Polyclonal Antibody

CAT#: TA364271

Rabbit polyclonal anti Prepro-Endothelin-1 (110-130) amide (po); neat antiserum

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against Prepro- Endothelin-1 (110-130) amide. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Reactivities: Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide H-Cys-Gln-Cys-Ala-Ser-Gln-Lys-Asp-Lys-Lys-Trp-Ser- Tyr-Cys-Gln-Ala-Gly-Lys-Glu-Ile-NH2 coupled to carrier protein.
• Formulation: Neat undiluted antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 50µl distilled water. This will give the equivalent of undiluted antiserum.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Database Link:
  UniProt ID P09558
• Background: Endothelin (ET-1) is a 21 amino acid peptide, also known as preproendothelin-1 (PPET1), that is produced by the vascular endothelium from a 39 amino acid precursor, big ET-1, through the actions of an endothelin converting enzyme (ECE) found on the endothelial cell membrane. ET-1 is involved in regulation of cardiovascular functions where it has been implicated in the pathogenesis of hypertension, coronary vasospasm, and heart failure. It is also known to causes fibrosis of the vascular cells as well as to stimulate production of reactive oxygen species. This antibody was generated by immunization of rabbits with Prepro-Endothelin-1 (110-130) amide coupled to a carrier protein.
• Synonyms: ET1; HDLCQ7; PPET1; Preproendothelin-1