Nps Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA |
Recommended Dilution | This antibody has been tested and validated in ELISA against Neuropeptide S. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user. |
Reactivities | Rat |
Host | Rabbit |
Clonality | Polyclonal |
Formulation | Each vial contains enough antiserum for 500 RIA tubes. The powder should be rehydrated with 50ml RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4°C. This should ensure antibody stability for approximately one month. |
Concentration | N/A |
Conjugation | Unconjugated |
Storage | Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below. |
Gene Name | neuropeptide S |
Database Link | |
Background | Neuropeptide S (NPS) is a neuropeptide found in human and mammalian brain, mainly produced by neurons in the amygdala and between Barrington's nucleus and the locus coeruleus, although NPS-responsive neurons extend projections into many other brain areas. It binds specifically to a G protein-coupled receptor, NPSR. Animal studies show that NPS suppresses anxiety and appetite, induces wakefulness and hyperactivity, including hyper-sexuality, and plays a significant role in the extinction of conditioned fear. It has also been shown to significantly enhance dopamine activity in the mesolimbic pathway, and inhibits motility and increases permeability in neurocrine fashion acting through NO in the myenteric plexus in rats and humans. This antibody was generated by immunization of rabbits with Neuropeptide S coupled to a carrier protein. |
Synonyms | ENSMUSG00000073804 |
Reference Data |
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