PAR-2 amide Rabbit Polyclonal Antibody

CAT#: TA364350

Rabbit polyclonal anti PAR-2 (1-6) amide (ms, rt); purified rabbit IgG

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: This antibody has been tested and validated in ELISA against PAR-2 (1- 6). Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide H-Ser-Leu-Ile-Gly-Arg-Leu-NH2 coupled to a carrier protein.
• Formulation: Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative.
• Concentration: N/A
• Conjugation: Unconjugated
• Storage: Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
• Background: Protease activated receptor 2 (PAR-2) is a proteolytically cleaved receptor which stimulates the increase of cytosolic Ca2+. SLIGKV-NH2 corresponds to the tethered ligand sequence of human PAR-2. PAR-2 (1-6) has been used as a proteinase-activated receptor 2 agonist to induce the expression of Hh and Hippo genes in Hek293A cells. This antibody was generated by immunization of rabbits with PAR-2 (1-6) coupled to a carrier protein.