MLLT3 Rabbit Polyclonal Antibody

CAT#: TA364584

MLLT3 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-3: HepG2 293T K562 cell lysates Primary antibody: TA364584 (MLLT3 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 2 minutes

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: HepG2, 293T, K562 cell lysates
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human MLLT3
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 63 kDa
• Database Link:
  UniProt ID P42568
• Background: Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated at 'Lys-9' (H3K9ac), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones.