ARFGAP1 Rabbit Polyclonal Antibody

CAT#: TA364606

ARFGAP1 rabbit polyclonal antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane 1-3: 293T cells PC3 cells NIH/3T3 cells Primary antibody: TA364606 (ARFGAP1 Antibody) at dilution 1/850 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: 293T, PC3 and NIH/3T3 cells
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human ARFGAP1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 45 kDa
• Database Link:
  UniProt ID Q8N6T3
• Background: The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.