NSMCE2 Rabbit Polyclonal Antibody

CAT#: TA365357

NSMCE2 rabbit polyclonal antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane: 293T cell Primary antibody: TA365357 (NSMCE2 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA365357 (NSMCE2 Antibody) at dilution 1/40 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA365357 (NSMCE2 Antibody) at dilution 1/40, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA365357 (NSMCE2 Antibody) at dilution 1/40 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA365357 (NSMCE2 Antibody) at dilution 1/40, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: 293T cell
  IHC: 30-150
  Positive control: Human lung cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length fusion protein
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 28 kDa
• Database Link:
  UniProt ID Q96MF7
• Background: NSMCE2 is the E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. NSMCE2 acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.