WASF1 Rabbit Polyclonal Antibody

CAT#: TA365428

WASF1 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-4: K562 NIH/3T3 U251 HUVEC cell lysates Primary antibody: TA365428 (WASF1 Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 10 seconds

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA365428 (WASF1 Antibody) at dilution 1/80 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA365428 (WASF1 Antibody) at dilution 1/80, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: K562, NIH/3T3, U251, HUVEC cell lysates
  IHC: 50-200
  Positive control: Human liver cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human WASF1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 62 kDa
• Database Link:
  UniProt ID Q92558
• Background: The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.