VASP Rabbit Polyclonal Antibody

CAT#: TA365437

VASP rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: A549 and HEPG2 cell lysates Primary antibody: TA365437 (VASP Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA365437 (VASP Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA365437 (VASP Antibody) at dilution 1/30, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: A549 and HEPG2 cell lysates
  IHC: 25-100
  Positive control: Human tonsil
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human VASP
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 40 kDa
• Database Link:
  UniProt ID P50552
• Background: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.