MED28 Rabbit Polyclonal Antibody

CAT#: TA365958

MED28 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: PC-3 cell lysate Primary antibody: TA365958 (MED28 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 10 seconds

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA365958 (MED28 Antibody) at dilution 1/240 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA365958 (MED28 Antibody) at dilution 1/240, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: PC-3 cell lysate
  IHC: 200-400
  Positive control: Human thyroid cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human MED28
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 19 kDa
• Database Link:
  UniProt ID Q9H204
• Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.