HLA-DPB1 Rabbit Polyclonal Antibody

CAT#: TA365989

HLA-DPB1 rabbit polyclonal antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-2: Raji cell and Human spleen tissue lysates Primary antibody: TA365989 (HLA-DPB1 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 seconds

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA365989 (HLA-DPB1 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA365989 (HLA-DPB1 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Raji cell and Human spleen tissue lysates
  IHC: 20-100
  Positive control: Human cervical cancer
  Predicted cell location: Cytoplasm and Cell membrane
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human HLA-DPB1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 29 kDa
• Database Link:
  UniProt ID P04440
• Background: HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.