ZAP70 Rabbit Polyclonal Antibody

CAT#: TA366300

ZAP70 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-4: Jurkat cell Mouse liver tissue HepG2 cell Mouse kidney tissue lysates Primary antibody: TA366300 (ZAP70 Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA366300 (ZAP70 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA366300 (ZAP70 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Jurkat cell, Mouse liver tissue, HepG2 cell, Mouse kidney tissue lysates
  IHC: 50-300
  Positive control: Human liver cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human ZAP70
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 70 kDa
• Database Link:
  UniProt ID P43403
• Background: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.