GSTM5 Rabbit Polyclonal Antibody

CAT#: TA366618

GSTM5 rabbit polyclonal antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-2: Mouse testis tissue Mouse liver tissue lysates Primary antibody: TA366618 (GSTM5 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 90 seconds

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA366618 (GSTM5 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA366618 (GSTM5 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Mouse testis tissue, Mouse liver tissue lysates
  IHC: 50-100
  Positive control: Human liver cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human GSTM5
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 26 kDa
• Database Link:
  UniProt ID P46439
• Background: Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds.