TP53 Rabbit Polyclonal Antibody

CAT#: TA366742

TP53(Ab-15) rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: 293T and 231 cell lysates Primary antibody: TA366742 (TP53(Ab-15) Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds

• 

  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA366742 (TP53(Ab-15) Antibody) at dilution 1/25 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA366742 (TP53(Ab-15) Antibody) at dilution 1/25, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: 293T and 231 cell lysates
  IHC: 20-100
  Positive control: Human thyroid cancer
  Predicted cell location: Nucleus and Cytoplasm
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human TP53
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 44 kDa
• Database Link:
  UniProt ID P04637
• Background: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants.