ACTH (POMC) Rabbit Polyclonal Antibody

CAT#: TA371974

POMC rabbit polyclonal antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-4: Hela 293T K562 and NIH/3T3 cell lysates Primary antibody: TA371974 (POMC Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA371974 (POMC Antibody) at dilution 1/25 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA371974 (POMC Antibody) at dilution 1/25, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Hela，293T，K562 and NIH/3T3 cell lysates
  IHC: 20-100
  Positive control: Human breast cancer
  Predicted cell location: Secreted
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human POMC
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 29 kDa
• Gene Name: proopiomelanocortin
• Database Link:
  Entrez Gene 5443 Human
  UniProt ID P01189
• Background: This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
• Synonyms: ACTH; adrenocorticotropin; alpha-MSH; beta-endorphin; beta-LPH; beta-MSH; CLIP; Corticotropin-lipotropin; gamma-LPH; gamma-MSH; LPH; met-enkephalin; MSH; NPP; OTTHUMP00000119991; OTTHUMP00000200964; POC; pro-ACTH-endorphin; proopiomelanocortin