B7H3 (CD276) Rabbit Polyclonal Antibody

CAT#: TA372195

CD276 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-4: PC-3 231 LNCAP and 293T cell lysates Primary antibody: TA372195 (CD276 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 15 seconds

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA372195 (CD276 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA372195 (CD276 Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: PC-3，231，LNCAP and 293T cell lysates
  IHC: 25-100
  Positive control: Human liver cancer
  Predicted cell location: Cytoplasm and Cell membrane
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human CD276
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 57 kDa
• Gene Name: CD276 molecule
• Database Link:
  Entrez Gene 80381 Human
  UniProt ID Q5ZPR3
• Background: The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Synonyms: 4Ig-B7-H3; B7-H3; B7H3