CHFR Rabbit Polyclonal Antibody

CAT#: TA372293

CHFR rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-6: Hela Jurkat A549 HepG2 293T and 231 cell lysates Primary antibody: TA372293 (CHFR Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA372293 (CHFR Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA372293 (CHFR Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Hela,Jurkat,A549,HepG2,293T and 231 cell lysates
  IHC: 20-100
  Positive control: Human ovarian cancer
  Predicted cell location: Nucleus
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human CHFR
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 73 kDa
• Gene Name: checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase
• Database Link:
  Entrez Gene 55743 Human
  UniProt ID Q96EP1
• Background: This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described.
• Synonyms: FLJ10796; FLJ33629; RNF116; RNF196