EHD3 Rabbit Polyclonal Antibody

CAT#: TA372455

EHD3 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Mouse brain tissue lysate Primary antibody: TA372455 (EHD3 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 minutes

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA372455 (EHD3 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA372455 (EHD3 Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Mouse brain tissue lysate
  IHC: 20-100
  Positive control: Human tonsil
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human EHD3
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 61 kDa
• Gene Name: EH domain containing 3
• Database Link:
  Entrez Gene 30845 Human
  UniProt ID Q9NZN3
• Background: The Eps15 homology (EH) domain-containing protein family consists of four members, EHD1, EHD2, EHD3, and EHD4. The chromosomal locations of the human EHD genes are as follows: EHD1 maps to 11q13, EHD2 maps to 19q13.3, EHD3 maps to 2p21, and EHD4 maps to 15q11.1 (1-3). The encoded proteins of all EHD family members contain multiple conserved regions, which include an amino-terminal nucleotide-binding consensus site, a bipartite nuclear localization signal, and a carboxy-terminal EH protein-binding domain with an EF-hand motif (3,4). EHD1 is ubiquitously expressed with increased expression in testis (2,3). EHD2, EHD3, and EHD4 have more specific expression with EHD2 highly expressed in heart, EHD3 expressed in brain, kidney, liver, placenta, ovary, and heart, and EHD4 expressed in heart, placenta, and pancreas (1,5,6). The EHD proteins may participate in ligand-induced endocytosis (3-5).
• Synonyms: EHD2; PAST3