elF2 alpha (EIF2S1) Rabbit Polyclonal Antibody

CAT#: TA372460

EIF2S1 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-6: A549 cell Human placenta tissue A172 cell NIH/3T3 cell Jurkat cell Hela cell lysates Primary antibody: TA372460 (EIF2S1 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 minutes

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA372460 (EIF2S1 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA372460 (EIF2S1 Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA372460 (EIF2S1 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA372460 (EIF2S1 Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: A549 cell,Human placenta tissue,A172 cell,NIH/3T3 cell,Jurkat cell,Hela cell lysates
  IHC: 30-150
  Positive control: Human cervical cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human EIF2S1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 36 kDa
• Gene Name: eukaryotic translation initiation factor 2 subunit alpha
• Database Link:
  Entrez Gene 1965 Human
  UniProt ID P05198
• Background: The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
• Synonyms: EIF-2; eIF-2-alpha; EIF-2A; EIF-2alpha; EIF2; EIF2A