HLA-DRB3 Rabbit Polyclonal Antibody

CAT#: TA372735

HLA-DRB3 rabbit polyclonal antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane: Raji cell lysate Primary antibody: TA372735 (HLA-DRB3 Antibody) at dilution 1/320 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 minutes

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA372735 (HLA-DRB3 Antibody) at dilution 1/25 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA372735 (HLA-DRB3 Antibody) at dilution 1/25, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 300-1000
  WB positive control: Raji cell lysate
  IHC: 10-50
  Positive control: Human tonsil
  Predicted cell location: Cell membrane
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human HLA-DRB3
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 30 kDa
• Gene Name: major histocompatibility complex, class II, DR beta 3
• Database Link:
  Entrez Gene 3125 Human
  UniProt ID P79483
• Background: HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB3 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
• Synonyms: HLA-DR3B