Topoisomerase I (TOP1) Rabbit Polyclonal Antibody

CAT#: TA372870

TOP1 rabbit polyclonal antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-2: 231 and K562 cell lysates Primary antibody: TA372870 (TOP1 Antibody) at dilution 1/900 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 2 minutes

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA372870 (TOP1 Antibody) at dilution 1/35 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA372870 (TOP1 Antibody) at dilution 1/35, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: 231 and K562 cell lysates
  IHC: 50-100
  Positive control: Human gastric cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human TOP1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 91 kDa
• Gene Name: topoisomerase (DNA) I
• Database Link:
  Entrez Gene 7150 Human
  UniProt ID P11387
• Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
• Synonyms: TOPI