STAT3 Rabbit Polyclonal Antibody

CAT#: TA372964

STAT3 rabbit polyclonal antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane: HepG2 cell lysate Primary antibody: TA372964 (STAT3 Antibody) at dilution 1/500 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 1 minute

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 200-1000
  WB positive control: HepG2 cell lysate
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human STAT3
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: 1 year
• Predicted Protein Size: 88 kDa
• Gene Name: signal transducer and activator of transcription 3
• Database Link:
  Entrez Gene 6774 Human
  UniProt ID P40763
• Background: The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is required for murine fetal development. Research studies have shown that Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding. Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways. Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage. It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain.
• Synonyms: APRF; FLJ20882; HIES; MGC16063