ACADS Rabbit Polyclonal Antibody

CAT#: TA373178

ACADS Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ACADS antibody (TA373178) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 15s.

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  Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug ACADS antibody (TA373178). Western blot was performed from the immunoprecipitate using ACADS antibody (TA373178) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 44kDa
• Gene Name: acyl-CoA dehydrogenase, C-2 to C-3 short chain
• Database Link:
  Entrez Gene 35 Human
  UniProt ID P16219
• Background: This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms.
• Synonyms: ACAD3; SCAD