ATP citrate lyase (ACLY) Rabbit Polyclonal Antibody

CAT#: TA373194

Phospho-ACLY-S455 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ACLY-S455 antibody (TA373194) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 60s.

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  Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells, using 3 ug Phospho-ACLY-S455 pAb (TA373194). Western blot was performed from the immunoprecipitate using Phospho-ACLY-S455 pAb (TA373194) at a dilition of 1:1000. NIH/3T3 cells were treated by Insulin (100 nM) at 37℃ for 10 minutes after serum-starvation overnight.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: WB,1:100 - 1:500
  IHC-P,1:50 - 1:100
  IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Mouse, Human, Rat
• Modifications: Phospho S455
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 121kDa
• Gene Name: ATP citrate lyase
• Database Link:
  Entrez Gene 47 Human
  UniProt ID P53396
• Background: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
• Synonyms: ACL; ATPCL; CLATP; OTTHUMP00000164773