alpha 1 Fetoprotein (AFP) Rabbit Polyclonal Antibody

CAT#: TA373341

Alpha-Fetoprotein (AFP) Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of mouse liver, using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) antibody (TA373341) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 60s.

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  Immunofluorescence analysis of human liver cancer using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) antibody (TA373341) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of HepG2 cells (upper left) and LO2 cells (negative sample control) (upper right) using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) Rabbit pAb (red, TA373341) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF/ICC,1:50 - 1:200
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 69kDa
• Gene Name: alpha fetoprotein
• Database Link:
  Entrez Gene 174 Human
  UniProt ID P02771
• Background: This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
• Synonyms: alpha-1-fetoprotein; Alpha-fetoglobulin; alpha-fetoprotein; FETA; HP; HPAFP; OTTHUMP00000160480