Argonaute 2 (AGO2) Rabbit Polyclonal Antibody

CAT#: TA373351

Argonaute-2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Argonaute-2 antibody (TA373351) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 20s.

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  Immunofluorescence analysis of L929 cells using Argonaute-2 Polyclonal Antibody (TA373351) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF/ICC,1:50 - 1:100
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 97kDa
• Gene Name: argonaute 2, RISC catalytic component
• Database Link:
  Entrez Gene 27161 Human
  UniProt ID Q9UKV8
• Background: This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: 1110029L17Rik; 2310051F07Rik; AI225898; AL022874; AW546247; Eif2c2; ENSMUSG00000072493; Gerp95