AKT1 Rabbit Polyclonal Antibody

CAT#: TA373418

Pan-Akt Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Pan-Akt antibody (TA373418) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunohistochemistry of paraffin-embedded rat ovary using Pan-Akt Rabbit pAb (TA373418) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded rat kidney using Pan-Akt Rabbit pAb (TA373418) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of C6 cells using AKT1/AKT2/AKT3 antibody (TA373418) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using AKT1/AKT2/AKT3 antibody (TA373418) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using AKT1/AKT2/AKT3 antibody (TA373418) at dilution of 1:100. Blue: DAPI for nuclear staining.

• Immunoprecipitation analysis of 25ug extracts of Rat brain cells using 3ug Pan-Akt antibody (TA373418). Western blot was performed from the immunoprecipitate using Pan-Akt antibody (TA373418) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
  IP,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human AKT1/AKT2/AKT3
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 48kDa/55kDa/51kDa/54kDa
• Gene Name: AKT serine/threonine kinase 1
• Database Link:
  Entrez Gene 207 Human
  UniProt ID P31749
• Background: Human AKT serine-threonine protein kinase family includes three members AKT1,AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.
• Synonyms: AKT; C-AKT; MGC99656; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; RAC-PK-alpha