ALK Rabbit Polyclonal Antibody

CAT#: TA373459

Phospho-ALK-Y1096 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HL-60 and HL-60 cells, using Phospho-ALK-Y1096 antibody (TA373459) at 1:1000 dilution. HL-60 cells were treated by 10% FBS after serum-starvation overnight. HL-60 cells were treated by UV for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:500 - 1:2000
• Reactivities: Human
• Modifications: Phospho Y1096
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around Y1096 of human ALK (NP_004295.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 176kDa
• Gene Name: anaplastic lymphoma receptor tyrosine kinase
• Database Link:
  Entrez Gene 238 Human
  UniProt ID Q9UM73
• Background: This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).
• Synonyms: CD246; NBLST3; TFG/ALK