Alkaline Phosphatase (ALPL) Rabbit Polyclonal Antibody

CAT#: TA373474

Alkaline Phosphatase (ALPL) Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of HeLa cells, using Alkaline Phosphatase (ALPL) antibody (TA373474) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IHC-P,1:50 - 1:200
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 57kDa
• Gene Name: alkaline phosphatase, liver/bone/kidney
• Database Link:
  Entrez Gene 249 Human
  UniProt ID P05186
• Background: This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
• Synonyms: AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; HOPS; MGC161443; MGC167935; OTTHUMP00000002972; TNAP; TNSALP