Aquaporin 2 (AQP2) Rabbit Polyclonal Antibody

CAT#: TA373616

AQP2 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using AQP2 antibody (TA373616) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunohistochemistry of paraffin-embedded mouse kidney using AQP2 antibody (TA373616) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of rat kidney cells using AQP2 antibody (TA373616) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of mouse kidney cells using AQP2 antibody (TA373616) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 147-271 of human AQP2 (NP_000477.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 28kDa
• Gene Name: aquaporin 2
• Database Link:
  Entrez Gene 359 Human
  UniProt ID P41181
• Background: This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus.
• Synonyms: AQP-2; AQP-CD; aquaporin-CD; MGC34501; WCH-CD