ASS1 Rabbit Polyclonal Antibody

CAT#: TA373754

ASS1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ASS1 antibody (TA373754) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

• 

  Immunofluorescence analysis of HeLa cells using ASS1 Rabbit pAb (TA373754) at dilution of 1:100. Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of NIH/3T3 cells using ASS1 Rabbit pAb (TA373754) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF/ICC,1:50 - 1:200
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 47kDa
• Gene Name: argininosuccinate synthase 1
• Database Link:
  Entrez Gene 445 Human
  UniProt ID P00966
• Background: The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
• Synonyms: ASS; CTLN1; OTTHUMP00000022363; OTTHUMP00000022364