ATG16L1 Rabbit Polyclonal Antibody

CAT#: TA373775

ATG16L1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ATG16L1 antibody (TA373775) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of H9C2 cells using ATG16L1 antibody (TA373775) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using ATG16L1 antibody (TA373775) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using ATG16L1 antibody (TA373775) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC-P,1:50 - 1:200
  IF/ICC,1:50 - 1:200
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 68kDa
• Gene Name: autophagy related 16 like 1
• Database Link:
  Entrez Gene 55054 Human
  UniProt ID Q676U5
• Background: The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.
• Synonyms: APG16L; ATG16L; FLJ00045; FLJ10035; FLJ10828; FLJ22677; IBD10; WDR30