ATP5A (ATP5A1) Rabbit Polyclonal Antibody

CAT#: TA373820

ATP5A1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ATP5A1 Antibody (TA373820) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunohistochemistry of paraffin-embedded rat ovary using ATP5A1 antibody (TA373820) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human liver cancer using ATP5A1 antibody (TA373820) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded human stomach using ATP5A1 antibody (TA373820) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse liver using ATP5A1 antibody (TA373820) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Confocal immunofluorescence analysis of Hela cells using ATP5A1 antibody (TA373820) at dilution of 1:200. Blue: DAPI for nuclear staining.

• Confocal immunofluorescence analysis of U-2OS cells using ATP5A1 antibody (TA373820) at dilution of 1:200. Blue: DAPI for nuclear staining.

• Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug ATP5A1 antibody (TA373820). Western blot was performed from the immunoprecipitate using ATP5A1 antibody (TA373820) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:100
  IP,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP5A1 (NP_004037.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 54kDa/57kDa/59kDa
• Gene Name: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
• Database Link:
  Entrez Gene 498 Human
  UniProt ID P25705
• Background: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
• Synonyms: ATP5A; ATP5AL2; ATPM; hATP1; MOM2; OMR; ORM