AXL Rabbit Polyclonal Antibody

CAT#: TA373880

Axl Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Axl antibody (TA373880) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

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  Immunofluorescence analysis of DU145 cells using Axl Rabbit pAb (TA373880) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of HeLa cells using Axl Rabbit pAb (TA373880) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Axl antibody (TA373880). Western blot was performed from the immunoprecipitate using AXL antibody (TA373880) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ELISA, ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IHC-P,1:50 - 1:200
  IF/ICC,1:50 - 1:200
  IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 98kDa
• Gene Name: AXL receptor tyrosine kinase
• Database Link:
  Entrez Gene 558 Human
  UniProt ID P30530
• Background: The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. The encoded protein acts as a host cell receptor for multiple viruses, including Marburg, Ebola and Lassa viruses and is a candidate receptor for the SARS-CoV2 virus.
• Synonyms: JTK11; UFO