B3GNT3 Rabbit Polyclonal Antibody

CAT#: TA373893

B3GNT3 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using B3GNT3 antibody (TA373893) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Immunohistochemistry of paraffin-embedded Human liver using B3GNT3 Rabbit pAb (TA373893) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded Rat ovary using B3GNT3 Rabbit pAb (TA373893) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC-P,1:50 - 1:200
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 43kDa
• Gene Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
• Database Link:
  Entrez Gene 10331 Human
  UniProt ID Q9Y2A9
• Background: This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. It plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking.
• Synonyms: B3GAL-T8; B3GALT8; B3GN-T3; B3GNT-3; Beta-3-Gx-T8; Beta3Gal-T8; Beta3Gn-T3; BGnT-3; Core1-beta3GlcNAcT; HP10328; TMEM3