CARD11 Rabbit Polyclonal Antibody

CAT#: TA374264

CARD11 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CARD11 antibody (TA374264) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

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  Immunofluorescence analysis of A549 cells using CARD11 antibody (TA374264). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF/ICC,1:50 - 1:100
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 133kDa
• Gene Name: caspase recruitment domain family member 11
• Database Link:
  Entrez Gene 84433 Human
  UniProt ID Q9BXL7
• Background: The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.
• Synonyms: BIMP3; CARMA1; MGC133069