PRMT4 (CARM1) Rabbit Polyclonal Antibody

CAT#: TA374269

PRMT4/CARM1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and PRMT4/CARM1 knockout (KO) 293T cells, using PRMT4/CARM1 antibody (TA374269) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

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  Western blot analysis of extracts of various cell lines, using PRMT4/CARM1 antibody (TA374269) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 15s.

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  Immunohistochemistry of paraffin-embedded human colon using [KO Validated] PRMT4/CARM1 Rabbit pAb (TA374269) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] PRMT4/CARM1 Rabbit pAb (TA374269) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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  Immunohistochemistry of paraffin-embedded rat kidney using [KO Validated] PRMT4/CARM1 Rabbit pAb (TA374269) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:200 - 1:2000
  IHC,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 409-608 of human PRMT4/CARM1 (NP_954592.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 41kDa/63kDa/65kDa
• Gene Name: coactivator associated arginine methyltransferase 1
• Database Link:
  Entrez Gene 10498 Human
  UniProt ID Q86X55
• Background: This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9.
• Synonyms: PRMT4