Caspase 9 (CASP9) Rabbit Polyclonal Antibody

CAT#: TA374293

Caspase-9 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (TA374293) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunohistochemistry of paraffin-embedded human placenta using Caspase-9 antibody (TA374293) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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  Immunofluorescence analysis of C6 cells using Caspase-9 antibody (TA374293) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 antibody (TA374293) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using Caspase-9 antibody (TA374293) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human Caspase-9 (NP_001220.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 17kDa/30kDa/36kDa/46kDa
• Gene Name: caspase 9
• Database Link:
  Entrez Gene 842 Human
  UniProt ID P55211
• Background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
• Synonyms: APAF-3; APAF3; CASP-9; CASPASE-9c; ICE-LAP6; MCH6