Caveolin 1 (CAV1) Rabbit Polyclonal Antibody

CAT#: TA374309

Phospho-Caveolin-1-Y14 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Phospho-Caveolin-1-Y14 Rabbit pAb (TA374309) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were treated by Hydrogen Peroxide (2 nM) at 37℃ for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 120s.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB,1:1000 - 1:2000
• Reactivities: Human, Mouse
• Modifications: Phospho Y14
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic phosphorylated peptide around Y14 of human Caveolin-1 (NP_001744.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 17kDa/20kDa
• Gene Name: caveolin 1
• Database Link:
  Entrez Gene 857 Human
  UniProt ID Q03135
• Background: The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.
• Synonyms: BSCL3; CAV; CGL3; MSTP085; VIP21