CHD4 Rabbit Polyclonal Antibody

CAT#: TA374719

CHD4 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CHD4 antibody (TA374719) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of C6 cells using CHD4 antibody (TA374719) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of L929 cells using CHD4 antibody (TA374719) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U20S cells using CHD4 antibody (TA374719) at dilution of 1:100. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC-P,1:100 - 1:200
  IF/ICC,1:50 - 1:200
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 218kDa
• Gene Name: chromodomain helicase DNA binding protein 4
• Database Link:
  Entrez Gene 1108 Human
  UniProt ID Q14839
• Background: The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Synonyms: CHD-4; DKFZp686E06161; Mi-2b; Mi2-beta