CREB1 Rabbit Polyclonal Antibody

CAT#: TA375073

CREB1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CREB1 Rabbit pAb (TA375073) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of NIH/3T3 cells using CREB1 Rabbit pAb (TA375073) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of PC-12 cells using CREB1 Rabbit pAb (TA375073) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U2OS cells using CREB1 Rabbit pAb (TA375073) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 300ug extracts of LO2 cells using 3ug CREB1 antibody (TA375073). Western blot was performed from the immunoprecipitate using CREB1 antibody (TA375073) at a dilition of 1:1000.

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Specifications

Product Data

• Applications: ELISA, ICC/IF, IHC, IP, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC-P,1:50 - 1:200
  IF/ICC,1:50 - 1:200
  IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 35kDa
• Gene Name: cAMP responsive element binding protein 1
• Database Link:
  Entrez Gene 1385 Human
  UniProt ID P16220
• Background: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
• Synonyms: CREB; CREB-1; MGC9284; OTTHUMP00000206660