CSDE1 Rabbit Polyclonal Antibody

CAT#: TA375128

CSDE1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts from normal (control) and CSDE1 knockout (KO) HeLa cells, using CSDE1 antibody (TA375128) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 15s.

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  Immunofluorescence analysis of L929 cells using CSDE1 Rabbit pAb (TA375128) at dilution of 1:100. Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug CSDE1 antibody (TA375128). Western blot was performed from the immunoprecipitate using CSDE1 antibody (TA375128) at a dilition of 1:1000.

Specifications

Product Data

• Applications: ELISA, ICC/IF, IP, WB
• Recommended Dilution: WB,1:500 - 1:1000
  IF/ICC,1:50 - 1:200
  IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 89kDa
• Gene Name: cold shock domain containing E1
• Database Link:
  Entrez Gene 7812 Human
  UniProt ID O75534
• Background: Enables RNA stem-loop binding activity. Involved in IRES-dependent viral translational initiation; nuclear-transcribed mRNA catabolic process, no-go decay; and stress granule assembly. Located in Golgi apparatus; cytosol; and plasma membrane. Part of CRD-mediated mRNA stability complex.
• Synonyms: D1S155E; DKFZp779B0247; DKFZp779J1455; FLJ26882; KIAA0885; NRAS-related; NRU; OTTHUMP00000013882; OTTHUMP00000013883; RP5-1000E10.3; UNR