Aromatase (CYP19A1) Rabbit Polyclonal Antibody

CAT#: TA375274

Aromatase (CYP19A1) Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Aromatase (CYP19A1) antibody (TA375274) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 20s.

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  Immunofluorescence analysis of HeLa cells using Aromatase (CYP19A1) antibody (TA375274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ELISA, ICC/IF, IHC, WB
• Recommended Dilution: WB,1:1000 - 1:2000
  IHC-P,1:50 - 1:200
  IF/ICC,1:50 - 1:200
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 58kDa
• Gene Name: cytochrome P450 family 19 subfamily A member 1
• Database Link:
  Entrez Gene 1588 Human
  UniProt ID P11511
• Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities.
• Synonyms: ARO; ARO1; Aromatase; CPV1; CYAR; CYP19; CYPXIX; MGC104309; OTTHUMP00000162543; P-450AROM