CYP27A1 Rabbit Polyclonal Antibody

CAT#: TA375281

CYP27A1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using CYP27A1 antibody (TA375281) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: WB,1:500 - 1:2000
  ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Formulation: Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 60kDa
• Gene Name: cytochrome P450 family 27 subfamily A member 1
• Database Link:
  Entrez Gene 1593 Human
  UniProt ID Q02318
• Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease.
• Synonyms: CP27; CTX; CYP27